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單細胞轉(zhuǎn)錄組測序(Single-cell RNA-sequencing, scRNA-seq)是在單個細胞水平對mRNA進行高通量測序的一項新技術(shù)，原理是將分離的單個細胞中微量的mRNA通過高效擴增后再進行高通量測序。

單細胞測序主要涉及單細胞基因組測序和轉(zhuǎn)錄組測序兩個方面，分別針對單細胞DNA及RNA進行序列分析和比較。單細胞測序一直是科學家關(guān)注的一個熱點。

Cells, the basic units of biological structure and function, vary broadly in type and state. In most biological systems, our knowledge of cellular diversity is incomplete, as for complex tissues like the nervous system (brain cells) [1].The

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Drop-seqDroplet Based scRNA-Seq Methods